rabbit polyclonal il 1 β  (Danaher Inc)

Journal: Cancers

Article Title: Study of Biological Effects Induced in Solid Tumors by Shortened-Duration Thermal Ablation Using High-Intensity Focused Ultrasound

doi: 10.3390/cancers16162846

Figure Lengend Snippet: Immunolabeling for IL-1α (red, ( left ) panel) and IL-1β (green, ( right ) panel) in a HIFU-treated tumor (3 days post-sonication) as compared to an untreated control tumor. White arrows indicate immunopositive cells. Bisbenzimide was used for visualization of cell nuclei (blue). Histograms show the intensity of immunolabeling for IL-α and IL1-β per microscopic field of view. The intensity of immunoreactivity is presented as the height of the surface plot profiles.

Article Snippet: Then, the sections were incubated for 1 h at 37 °C with PBS+T containing 1% normal goat serum and one or two of the following primary antibodies (Abs): (1) murine monoclonal Ab against vascular endothelial growth factor (VEGF) (Santa Cruz Biotechnology, Santa Cruz, CA, USA, dil. 1:400); (2) rabbit polyclonal Ab against IL 1-β (Invitrogen-Molecular Probes, Eugene, OR, USA, dil. 1:400); (3) murine monoclonal Ab against IL 1-α (Santa Cruz Biotechnology, CA, USA, dil. 1:400); (4) murine monoclonal Ab against TNF-α (Santa Cruz Biotechnology, CA, USA, dil. 1:400); (5) murine monoclonal Ab against macrophages (Santa Cruz Biotechnology, CA, USA, dil. 1:400); (6) murine monoclonal Ab against lymphocytes CD8 (DAKO, Santa Clara, CA, USA, dil. 1:400), and (7) murine monoclonal Ab against INF-γ (Santa Cruz Biotechnology, CA, USA, dil. 1:400).

Techniques: Immunolabeling, Sonication, Control

Journal: Journal of Inflammation Research

Article Title: Class IIa HDAC Downregulation Contributes to Surgery-Induced Cognitive Impairment Through HMGB1-Mediated Inflammatory Response in the Hippocampi of Aged Mice

doi: 10.2147/JIR.S304060

Figure Lengend Snippet: Early peripheral-inflammatory response and neuroinflammation following surgery or LMK235 treatment. ( A-C ) The serum level of IL-1 β , IL-6, and TNF- α on postoperative day 1, 3, and 7 were measured by ELISA, n = 4 per group. Each sample repeated the experiment 3 times. ( D–F ) Representative Western blots of TNF- α and IL-1 β on postoperative days 1, 3, and 7, n = 4 per group. Each sample repeated the experiment 3 times. ( G, H ) Densitometric analysis of Western blot data from D–F, n = 4 per group. #p < 0.05 versus the day-matched control group. *p < 0.05 versus the day-matched anesthesia group.

Article Snippet: A TBS buffer of 10% non-fat dried milk blocked membranes for 2 hours and incubated with primary antibodies: rabbit anti-HMGB1 monoclonal (1:3000; ab-18256; Abcam, Inc., MA, USA), rabbit anti-RAGE polyclonal (1:1000; DF6618; Affinity, UK), rabbit anti-TNF- α polyclonal (1:1000; sc-12744; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), rabbit anti-IL-1 β polyclonal (1:1000; sc-7884; Santa Cruz Biotechnology Inc.), rabbit anti-HDAC4 (1:2000; ab-79521; Abcam, Cambridge, UK), rabbit anti-HDAC5 polyclonal (1:2000; sc-133225; Santa Cruz Biotechnology Inc.), rabbit anti-TLR4 polyclonal (1:1000; sc-293072; Santa Cruz Biotechnology Inc.), and mouse anti-MyD88 monoclonal antibody (1:1000; sc-74532; Santa Cruz Biotechnology Inc.) overnight at 4 °C.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot